Amino Acid Carriers of the Solute Carrier Families 7 (SLC7) and 38 (SLC38) Are Involved in Leucine Sensing in the Brain of Atlantic Salmon (Salmo salar)

Sensing of amino acids in fish brain, especially branched-chain (BCAA) like leucine, is involved regulation feed intake through different mechanisms. However, there limited information regarding the possible involvement mechanisms dependent on acid carriers solute carrier families (SLC) known to be key regulators intracellular leucine concentration, namely L-type transporter 1 (LAT1), and sodium-dependent neutral 2 (SNAT2) 9,(SNAT9), for which evidence their participation available mammals. Comparative analysis amongst sequences revealed a complex pattern paralogues Atlantic salmon, LAT1 ( slc7a5aa , slc7a5ab slc7a5ba slc7a5bb slc7a5ca slc7a5cb ), SNAT2 slc38a2a slc38a2b ) SNAT9 slc38a9 ). After establishing phylogenetic relationships evaluated, samples selected brain areas were taken from salmon assess tissue distribution transcripts. In an additional experiment, fed two diets with levels (high leucine: 35 g/kg vs. control 27.3 g/kg). The high diet resulted lower increased mRNA abundance specific slc7a5aa, though apparently not hypothalamus telencephalon food regulation. results obtained suggest role members SLC family anorectic effect thus as sensing mechanism characterised so far intake.